Somatotropin (Protein name
), SOMA_BOVIN from NCBI database.
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General Annotation
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Antigen Annotation
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3D
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Predicted Eptitope
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Vaild Sequence
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Gene name:
GH1(GH);
Protein name:
Somatotropin;
Alternative:
Growth hormone;
Organism:
Bovine (Bos taurus).
General Annotation
Sub Unit:
N/A
Function:
Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
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s
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Precision
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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤4.5%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤7.3%
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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤4.0%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤7.5%
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Recovery
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Recovery was determined by spiking various levels of GH1 into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
108
105-110
Plasma
109
107-112
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Recovery was determined by spiking various levels of GH1 into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
99
98-105
Plasma
98
96-103
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Linearity
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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GH1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1：2
1:4
1:8
1:16
serum(n=5)
105-110%
89-95%
97-106%
103-114%
EDTA plasma(n=5)
103-108%
109-113%
112-115%
96-105%
heparin plasma(n=5)
89-99%
109-115%
99-105%
106-112%
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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GH1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1：2
1:4
1:8
1:16
serum(n=5)
96-100%
87-95%
98-103%
106-109%
EDTA plasma(n=5)
97-99%
95-108%
103-110%
94-103%
heparin plasma(n=5)
97-106%
94-99%
89-96%
96-104%
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Cited for: NUCLEOTIDE SEQUENCE [MRNA]
6.
"The complete sequence of a cDNA encoding the bovine growth hormone." Mauro S.M.Z.
,
Ferro M.I.T.
,
Macari M.
,
Ferro J.A.
Submitted (1997-11) to the EMBL/GenBank/DDBJ databases
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Cited for: NUCLEOTIDE SEQUENCE [MRNA]
"Cloning and sequencing of the growth hormone gene of Pakistani cow breeds (Bos taurus)." Mahmood S.F.
,
Awan I.N.
,
Khan M.J.
,
Shahzad M.I.
,
Khanum A.
Submitted (2007-02) to the EMBL/GenBank/DDBJ databases
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Cited for: NUCLEOTIDE SEQUENCE [MRNA]
Vaild Sequence:
